Preparation method of agonist for improving fish sperm motility

The invention relates to a preparation method of an agonist for improving fish sperm motility. The method comprises the following steps: (1) taking the fish gonads in a development stage V; (2) separating interstitial cells of the fish gonad; (3) adding an HHBS cell culture solution; (4) adding 3mL of hyaluronidase into a centrifugal tube, adding 5 mL of the cell culture solution after concussion,and obtaining a supernatant by centrifugation; (5) filtering the supernatant with a cell sieve; (6) respectively adding DMSO, glutathione and cysteine to the filtered cell supernatant, blowing, beating, uniformly mixing, and carrying out cryopreservation at the temperature of -20 DEG C so as to obtain the product. The method has the advantages of good application effect, low cost and easiness inprocessing and production. 本发明涉及一种提高鱼类精子活力激动剂的制备方法，包括以下步骤：(1)取性腺处于发育V期的鱼类性腺(2)分离鱼类性腺的间质细胞(3)加入HHBS细胞培养液(4)在离心管中加入3mL透明质酸酶，震荡后加入5mL细胞培养液，离心取上清(5)将上清液用细胞筛过滤(6)将过滤后的细胞上清液，分别添加DMSO，谷胱甘肽、半胱氨酸，吹打混匀，放到-20℃冷冻保存，即得。本发明的优点是应用效果好、成本低、容易加