Kit and detection method capable of synchronously detecting African swine fever virus antigens and antibodies in swine blood

The invention discloses a kit and detection method capable of synchronously detecting African swine fever virus antigens and antibodies in swine blood, and belongs to the technical field of biology. According to the kit provided by the invention, a detection line for detecting African swine fever virus antigens and antibodies is arranged on an analysis membrane of a colloidal gold duplex card at the same time; preferably, the African swine fever virus P30 protein is used as an antibody for detection, the monoclonal antibody of the African swine fever virus P72 protein is used as an antigen fordetection, and the matched sample diluent is matched for use, so the African swine fever virus P30 protein antibody and the P72 protein antigen in the whole blood of the pig can be simultaneously detected through one-step operation. 本发明公开了一种能够同步检测猪血中非洲猪瘟病毒抗原和抗体的试剂盒及检测方法，属于生物技术领域。本发明提供的试剂盒在胶体金二联卡的分析膜上同时设置检测非洲猪瘟病毒抗原和抗体的检测线，并优选非洲猪瘟病毒P30蛋白做抗体的检测，非洲猪瘟病毒P72蛋白的单克隆抗体做抗原的检测，配合使用配套的样品稀释液，可以一步法操作同时检测猪全血中非洲猪瘟病毒P30蛋白抗体和P72蛋白抗原。