Digital PCR detection method and test kit for novel coronavirus nucleic acid

The invention provides a digital PCR detection method and a test kit for novel coronavirus, in particular to a dual digital PCR detection system designed for a novel coronavirus ORF1ab gene and an N gene. Experimental results show that the dual digital PCR detection system disclosed by the invention...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	XU XIAOJIE, JIANG XIWEN, PAN CUIYUAN, HUANG ZHIWEN, ZHU XIAOYA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a digital PCR detection method and a test kit for novel coronavirus, in particular to a dual digital PCR detection system designed for a novel coronavirus ORF1ab gene and an N gene. Experimental results show that the dual digital PCR detection system disclosed by the invention has extremely high sensitivity and accuracy. 本发明提供了新型冠状病毒的数字PCR检测方法及试剂盒，具体地本发明针对新型冠状病毒ORF1ab基因和N基因设计了双重数字PCR检测体系，实验结果表明本发明的双重数字PCR检测体系具有极高的灵敏度和准确性。