Identification method and application of heparin and heparan sulfate

The invention provides an identification method and application of heparin and heparan sulfate, and belongs to the technical field of medicine detection. Research finds that eight disaccharide components of 0S, NS, 6S, 2S, 2SNS, 6SNS, 2S6S and TriS are rich in heparin drugs, and can be well used for identifying heparin and heparan sulfate as a mixed standard substance. Therefore, a set of quantitative detection method based on hydrophilic interaction chromatography-mass spectrometry is established for the eight components, and the method has the advantages of being easy and convenient to operate, short in consumed time, high in selectivity, high in sensitivity, good in specificity, good in repeatability and the like. The method can be used for simply, conveniently and accurately identifying heparin and heparan sulfate, a method and data reference are provided for constructing a more complete quality control standard system of glycosaminoglycan drugs, so that the method has good practical application value. 本发