IMPROVED METHOD FOR MEASUREMENT OF MICROBIAL BIOMASS

The invention relates to a method for quantitative or qualitative determination of at least one fungus of interest in a sample where use is made of an assay that determines enzymatic activity derivedfrom the fungus. In addition, an enzyme inhibitor that preferentially inhibits the corresponding enzymatic activity in other cells potentially present in the sample is added so as to minimize false positive enzyme activity measurements from these other cells. Also a kit comprising a substrate for the enzyme to be determined and an inhibitor of the enzyme activity is provided. Moreover, the invention also relates to a method for determination of relative concentrations/numbers of fungal cells in samples comprising other biomass. 本发明涉及一种用于定量或定性测定样品中至少一种感兴趣的真菌的方法，其中使用了测定来源于真菌的酶活性的测定。此外，添加了优先抑制样品中潜在存在的其他细胞中的相应酶活性的酶抑制剂，以使来自这些其他细胞的假阳性酶活性测量最小化。还提供了一种试剂盒，其包含待测定的酶的底物和酶活性的抑制剂。此外，本发明还涉及一种用于测定包含其他生物质的样品中的真菌细胞的相对浓度/数量的方法。