Separation and detection method of Baloxavir marboxil optical isomer

The invention relates to a separation and detection method of an optical isomer, and belongs to the field of analytical chemistry. The method specifically relates to separation and determination of Baloxavir marboxil and optical isomers thereof by liquid chromatography, and is characterized in that a polysaccharide derivative is used as a chiral chromatographic column of a filler, and a mixed solution of lower alkane and lower alcohol is used as a mobile phase. The method is simple, rapid and accurate. 本发明涉及一种光学异构体的分离检测方法，属于分析化学领域。所述方法具体涉及用液相色谱法分离测定Baloxavir marboxil及其光学异构体，其特征在于，以多糖衍生物为填料的手性色谱柱，以低级烷烃与低级醇的混合溶液为流动相。本发明的方法简单、快速、准确。