Preparation method of molecular diagnosis enzyme preparation

The invention discloses a preparation method of a molecular diagnosis enzyme preparation. The preparation method comprises the following steps: 1) constructing an expression strain: 1-1) cloning a nucleic acid sequence of a selected expression gene; 1-2) constructing a recombinant plasmid; 1-3) obtaining a recombinant expression strain; 2) performing target protein expression on the recombinant expression strain; and 3) purifying target protein. According to the preparation method of the molecular diagnosis enzyme preparation, high-purity DNA polymerase can be obtained, can be used for a common PCR reaction, and can also be effectively used in fluorescent quantitative PCR and digital PCR reactions with high requirements on reaction conditions; the purification method disclosed by the invention can be applied to purification processes of all heat-resistant DNA polymerases, the operation difficulty of related experiments can be reduced, and reference and help can be provided for industrial large-scale productio