Stable vaccine compositions comprising inter alia live attenuated recombinant flavivirus and process for preparation thereof

Stable lyophilized immunogenic compositions comprising inter alia live attenuated recombinant flaviviruses, more preferably live attenuated recombinant dengue viruses, at least one carbohydrate, at least one amino acid and particularly amenable to rapid freeze-drying treatments. The composition preserves desired characteristics of a virus, including virus viability, immunogenicity and stability. The said immunogenic composition is devoid of preservatives, polymers and surfactants. The methods for manufacturing said stable lyophilized immunogenic compositions are provided. 一种稳定的冻干免疫原性组合物，其尤其包含减毒活重组黄病毒，更优选地减毒活重组登革热病毒、至少一个碳水化合物、和至少一个氨基酸，并且其特别适于快速冷冻干燥处理，其中，该组合物保留了病毒的期望特性，期望特性包括病毒生存力、免疫原性和稳定性。所述免疫原性组合物不含防腐剂、聚合物和表面活性剂。一种用于制造所述稳定的冻干免疫原性组合物的方法。