gRNA capable of specifically recognizing porcine COL1A1 gene and biological materials of gRNA, kit and application

The invention relates to the field of genetic engineering, and particularly provides gRNA capable of specifically recognizing a porcine COL1A1 gene and biological materials of gRNA, a kit and application. In the gRNA capable of specifically recognizing the porcine COL1A1 gene, a nucleotide sequence responsible for recognizing a target fragment region is shown as SEQ ID NO.1. The gRNA can recognizethe downstream of a coding region of the porcine COL1A1 gene such as a targe sequence shown as SEQ ID NO.5. Through the mediation of the gRNA, Cas9 can target the tail end of the 51 exon of the COL1A1 gene on a porcine genome to produce a double-strand breaking, the cutting efficiency can reach 22%, then the editing of the COL1A1 gene is realized, such as amino acid modification and replacement, or insertion of a foreign gene expression box at a site, and stable and efficient expression of foreign genes is achieved. 本发明涉及基因工程领域，具体而言，提供了一种特异性识别猪COL1A1基因的gRNA及其生物材料、试剂盒和应用。特异性识别猪COL1A1基因的gRNA中负责识别靶片段区域的核苷酸序列为SEQ ID NO.1