RAA primer, probe and detection method for detecting pasteurella multocida diseases

The invention discloses a primer, probe and detection method for detecting pasteurella multocida by an RAA fluorescence method. The primer and the probe are suitable for detection by the RAA fluorescence method, can accurately detect pasteurella multocida plasmids and positive samples, and do not have cross reactions with mycoplasma, streptococcus, escherichia coli, eperythrozoon, neospora caninum, toxoplasma gondii and healthy cow blood, and the specificity reaches 100%. The detection method is quick, and high flux is easy to realize, besides, the detection time is reduced, and the detectioncost is reduced. A method for quickly detecting pasteurella multocida DNA based on the RAA fluorescence method, provided by the invention, is high in sensitivity, and the reaction detection sensitivity reaches 10copies/[mu] L. 本发明公开了一种RAA荧光法检测多杀性巴氏杆菌的引物、探针及检测方法，其中引物和探针适用于RAA荧光法检测，并且能够准确地检测出多杀性巴氏杆菌质粒和阳性样本，与支原体、链球菌、大肠杆菌、附红细胞体、新孢子虫、弓形虫、健康牛血液无交叉反应，特异性达100％；检测方法快速、容易实现高通量，同时降低了检测时间和检测成本，本发明提供的基于RAA荧光法快速检测多杀性巴氏杆菌DNA的方法，灵敏度高，每反应