De-host extraction kit for phlegm macrogenome

The invention provides a de-host extraction method for a sputum pathogenic microorganism metagenome. The method comprises steps of sputum pretreatment, differential lysis to remove the host and nucleic acid extraction. The invention combines DTT and protease to quickly homogenize sputum, and then us...

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Bibliographische Detailangaben
Hauptverfasser:	LIU SISI, WANG XIAONAN, SHAO YANG, LIU JIA, ZHAO MINCHAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a de-host extraction method for a sputum pathogenic microorganism metagenome. The method comprises steps of sputum pretreatment, differential lysis to remove the host and nucleic acid extraction. The invention combines DTT and protease to quickly homogenize sputum, and then uses surfactant to differentially lyse cells, reduces interference of human genes, realizes enrichmentof microbial genome, and effectively improves detection rate of metagenomic pathogens in sputum samples. 本发明提出一种痰液病原微生物宏基因组的去宿主化提取方法，包括痰液前处理液、差异裂解去宿主和核酸提取步骤。本发明联用DTT与蛋白酶快速匀质化痰液，后采用表面活性剂差异裂解细胞，降低人源基因的干扰，实现微生物基因组的富集，有效提升痰标本宏基因组病原检出率。