Method for obtaining regenerated plant by utilizing radix saposhnikoviae leaves

The invention discloses a method for obtaining a regenerated plant by utilizing radix saposhnikoviae leaves. The method comprises the following steps of 1) performing culturing on a radix saposhnikoviae explant to obtain a callus, 2) carrying out induced culture on the callus to obtain a somatic embryo, and 3) carrying out further culturing on the somatic embryo to obtain the radix saposhnikoviaeregenerated plant. The key point of the test is to obtain a set of scheme for rapidly utilizing leaves and obtaining regenerated seedlings through a somatic embryo way, and the number of the obtainedregenerated seedlings can be controlled by controlling the number of subculture multiplication cells. 本发明公开了一种利用防风叶片获得再生植株的方法，包括：1)将防风外植体培养得到愈伤组织，2)将愈伤组织进行诱导培养得到体细胞胚胎，3)将所述体细胞胚胎进一步培养得到防风再生植株。本发明的试验关键点是获得一套快速利用叶片，通过体细胞胚胎途径获得再生苗的方案，所获再生苗的数量可通过控制继代增殖细胞的数量控制。