Tumor single cell detection kit

The invention provides a tumor single cell detection kit, which is used for obtaining tumor single cells from body fluid, and is characterized by comprising: lipid magnetic nano microspheres used forcapturing tumor cells from the body fluid; the cell staining solution that is used for staining tumor...

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Bibliographische Detailangaben
Hauptverfasser: LIANG XIAOFEI, GAO TIANWEI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a tumor single cell detection kit, which is used for obtaining tumor single cells from body fluid, and is characterized by comprising: lipid magnetic nano microspheres used forcapturing tumor cells from the body fluid; the cell staining solution that is used for staining tumor cells; the glass slide that is used for containing the tumor cells subjected to gradient dilution,and comprises a glass slide main body and a plurality of sample loading holes formed in the glass slide main body, wherein antibodies corresponding to tumor cells are arranged on the surface of the lipid magnetic nano-microsphere, the sample loading holes are surrounded by super-hydrophobic ink coated on the surface of the glass slide main body, and the sample loading amount of the sample loadingholes is 1-100 mu L. By means of the detection kit, tumor single cells can be efficiently and rapidly separated and obtained with low cost, and the obtained tumor single cells can be used for singlecell genome sequencing and t