Artificial simulated nucleic acid molecular beacons and kit for detecting rs12041331 site polymorphism of PEAR1 gene

The invention discloses a typing detection method and kit for rs12041331 site polymorphism of a PEAR1 gene. According to the invention, PEAR1 gene specific primers SEQ1 and SEQ2 are adopted to amplifyPEAR1 gene segments, and PEAR1 gene specific artificial simulated nucleic acid molecular beacons SEQ3-FAM and SEQ4-VIC are designed in an amplification area defined by the PEAR1 gene specific primers. The method for judging the rs12041331 site polymorphism of the PEAR1 gene based on the combination of the gene specific PCR and the artificial simulated nucleic acid molecular beacons provided by the invention not only has high accuracy rate, but also has the advantages of high detection speed, simple operation, objective result interpretation, less closed tube reaction pollution and the like, and is very suitable for large-scale clinical development. 本发明公开了一种PEAR1基因rs12041331位点多态性的分型检测方法与试剂盒。本发明采用PEAR1基因特异性的引物SEQ1和SEQ2扩增PEAR1基因片段，同时在PEAR1基因特异性的引物界定的扩增区域内设计PEAR1基因特异性人工模拟核酸分子信标SEQ3-FAM和SEQ4-VIC。本发明提供的基于基因特异性PCR结合人工模拟