Artificial simulated nucleic acid molecular beacons and kit for detecting rs4880 site polymorphism of SOD2 gene
The invention discloses a typing detection method and kit for rs4880 site polymorphism of an SOD2 gene. According to the invention, SOD2 gene specific primers SEQ1 and SEQ2 are adopted to amplify SOD2gene fragments, and SOD2 gene specific artificial simulated nucleic acid molecular beacons SEQ3-FAM...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a typing detection method and kit for rs4880 site polymorphism of an SOD2 gene. According to the invention, SOD2 gene specific primers SEQ1 and SEQ2 are adopted to amplify SOD2gene fragments, and SOD2 gene specific artificial simulated nucleic acid molecular beacons SEQ3-FAM and SEQ4-VIC are designed in an amplification region defined by the SOD2 gene specific primers. Themethod for judging the rs4880 site polymorphism of the SOD2 gene based on the combination of the gene specific PCR and the artificial simulated nucleic acid molecular beacons provided by the invention not only has high accuracy rate, but also has the advantages of high detection speed, simple operation, objective result interpretation, less closed tube reaction pollution and the like, and is verysuitable for large-scale clinical development.
本发明公开了一种SOD2基因rs4880位点多态性的分型检测方法与试剂盒。本发明采用SOD2基因特异性的引物SEQ1和SEQ2扩增SOD2基因片段,同时在SOD2基因特异性的引物界定的扩增区域内设计SOD2基因特异性人工模拟核酸分子信标SEQ3-FAM和SEQ4-VIC。本发明提供的基于基因特异性PCR结合人工模拟核酸分子信标判断SOD2基因rs4880位 |
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