Fluorescent cell automatic counting method, device, equipment and medium

The invention provides a fluorescent cell automatic counting method and device, equipment and a medium. The method comprises the following steps: S1, reading a microscopic image of fluorescent cells;S2, preprocessing the microscopic image of the fluorescent cell, including correcting uneven illumination of the microscopic image and enhancing the contrast between the cell in the microscopic imageand the background; adopting median filtering to filter microscopic image noise, and reserving detail information of the edge of the image; removing microscopic image noise and background impurities by adopting opening operation, smoothing cell contours, disconnecting cell stenosis connection and removing small protruding parts of cells; s3, segmenting the fluorescent cells adhered to the microscopic image to prevent the adhered cell population from serving as one cell during cell counting; and S4, calculating the number of the fluorescent cells. Requirements of most laboratories can be met from the aspects of efficien