Kit and method for detecting gene mutation of acute lymphoblastic leukemia based on multiplex-PCR targeted high-throughput sequencing

The invention discloses a kit for detecting gene mutation of acute lymphoblastic leukemia based on multiplex-PCR targeted high-throughput sequencing and a detection method of the kit. The kit is usedfor detecting multiple exon regions of 19 genes related with the acute lymphoblastic leukemia. The kit contains a primer working solution for detecting relevant gene mutation, a PCR mixed solution, aPCR reaction solution, a digestion solution, a joint P5 and a joint P7. The kit is used for carrying out detection based on a multiplex-PCR targeted high-throughput sequencing technique, has the advantages of high accuracy, sensitivity and throughput and can be used for rapidly and efficiently carrying out standardized quantitative detection on 19 relevant genes of acute lymphoblastic leukemia (ALL). 本发明公开了一种基于多重PCR的靶向高通量测序的急性淋巴细胞白血病基因突变检测试剂盒及其检测方法。所述的试剂盒用于检测急性淋巴细胞白血病相关的19种基因的多个外显子区域。所述的试剂盒包括检测相关基因突变的引物工作液，PCR混合液、PCR反应液、消化液、接头P5及接头P7。该试剂盒是基于多重PCR的靶向高通量测序技术进行的检测，具有高准确性、高灵敏度及高通量的优势，能快速高效对19种急性淋巴细胞白血病(ALL)相关基因进行标准化的定量检测。