Cell strain for stably expressing NFAT, synthesis method and application of cell strain
The invention belongs to the technical field of bioengineering, and discloses a cell strain for stably expressing NFAT, a synthesis method and application of the cell strain. The synthesis method of the cell strain for stably expressing NFAT comprises the following steps: cell subculture, preparatio...
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| creator | LIU SHENG ZHANG HAITAO PAN ZHENGBO |
| description | The invention belongs to the technical field of bioengineering, and discloses a cell strain for stably expressing NFAT, a synthesis method and application of the cell strain. The synthesis method of the cell strain for stably expressing NFAT comprises the following steps: cell subculture, preparation of a transfection reagent, transfection, continuous culture, amplification, screening and identification. The synthesis method of the cell strain for stably expressing NFAT can be used for obtaining the cell strain for stably expressing NFAT, is simple and is high in universality. Meanwhile, by the system, candidate cell data are utilized to carry out feature acquisition on images of a cell image layer so as to obtain a cell membrane feature value and a cell nucleus feature value, the candidate cells are classified into target cells and non-target cells according to the cell membrane feature value and the cell nucleus feature value, and the cells are classified according to the specific morphology of the cells so |
| format | Patent |
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The synthesis method of the cell strain for stably expressing NFAT comprises the following steps: cell subculture, preparation of a transfection reagent, transfection, continuous culture, amplification, screening and identification. The synthesis method of the cell strain for stably expressing NFAT can be used for obtaining the cell strain for stably expressing NFAT, is simple and is high in universality. Meanwhile, by the system, candidate cell data are utilized to carry out feature acquisition on images of a cell image layer so as to obtain a cell membrane feature value and a cell nucleus feature value, the candidate cells are classified into target cells and non-target cells according to the cell membrane feature value and the cell nucleus feature value, and the cells are classified according to the specific morphology of the cells so</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CALCULATING ; CHEMISTRY ; COMPOSITIONS THEREOF ; COMPUTING ; COUNTING ; CULTURE MEDIA ; ENZYMOLOGY ; HANDLING RECORD CARRIERS ; IMAGE DATA PROCESSING OR GENERATION, IN GENERAL ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PRESENTATION OF DATA ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; RECOGNITION OF DATA ; RECORD CARRIERS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200825&DB=EPODOC&CC=CN&NR=111575318A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200825&DB=EPODOC&CC=CN&NR=111575318A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU SHENG</creatorcontrib><creatorcontrib>ZHANG HAITAO</creatorcontrib><creatorcontrib>PAN ZHENGBO</creatorcontrib><title>Cell strain for stably expressing NFAT, synthesis method and application of cell strain</title><description>The invention belongs to the technical field of bioengineering, and discloses a cell strain for stably expressing NFAT, a synthesis method and application of the cell strain. The synthesis method of the cell strain for stably expressing NFAT comprises the following steps: cell subculture, preparation of a transfection reagent, transfection, continuous culture, amplification, screening and identification. The synthesis method of the cell strain for stably expressing NFAT can be used for obtaining the cell strain for stably expressing NFAT, is simple and is high in universality. 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| subjects | BEER BIOCHEMISTRY CALCULATING CHEMISTRY COMPOSITIONS THEREOF COMPUTING COUNTING CULTURE MEDIA ENZYMOLOGY HANDLING RECORD CARRIERS IMAGE DATA PROCESSING OR GENERATION, IN GENERAL METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PRESENTATION OF DATA PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOGNITION OF DATA RECORD CARRIERS SPIRITS VINEGAR WINE |
| title | Cell strain for stably expressing NFAT, synthesis method and application of cell strain |
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