Cell strain for stably expressing NFAT, synthesis method and application of cell strain

The invention belongs to the technical field of bioengineering, and discloses a cell strain for stably expressing NFAT, a synthesis method and application of the cell strain. The synthesis method of the cell strain for stably expressing NFAT comprises the following steps: cell subculture, preparatio...

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Bibliographische Detailangaben
Hauptverfasser: LIU SHENG, ZHANG HAITAO, PAN ZHENGBO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of bioengineering, and discloses a cell strain for stably expressing NFAT, a synthesis method and application of the cell strain. The synthesis method of the cell strain for stably expressing NFAT comprises the following steps: cell subculture, preparation of a transfection reagent, transfection, continuous culture, amplification, screening and identification. The synthesis method of the cell strain for stably expressing NFAT can be used for obtaining the cell strain for stably expressing NFAT, is simple and is high in universality. Meanwhile, by the system, candidate cell data are utilized to carry out feature acquisition on images of a cell image layer so as to obtain a cell membrane feature value and a cell nucleus feature value, the candidate cells are classified into target cells and non-target cells according to the cell membrane feature value and the cell nucleus feature value, and the cells are classified according to the specific morphology of the cells so