Method for inducing embryogenic callus of test tube corms of Jiangxi Yanshan Colocasia esulenla Schott

The invention discloses a method for inducing embryogenic callus of test tube corms of Jiangxi Yanshan Colocasia esulenla Schott, and belongs to the technical field of callus culture. The method comprises the following steps: taking test tube corms induced byJiangxi Yanshan Colocasia esulenla Schott as explants, slicing the test tube corms subjected to two-time pretreatment, embedding the sliced test tube corms to obtain test tube corm slice embedded beads, and then inoculating the embedded beads to solid culture media with different nutritional ingredients for culture so that the embryogeniccallus of the test tube corm slices of Jiangxi Yanshan Colocasia esulenla Schott can be induced. By the callus induction method disclosed by the invention, the induction rate of the embryogenic callusis as high as 95% or above, and the regeneration rate of the embryogenic callus can be 100%. 本发明公开了一种江西铅山红芽芋试管球茎胚性愈伤组织诱导的方法，属于愈伤组织培养技术领域。本发明以江西铅山红芽芋诱导的试管球茎作为外植体，经过两次预处理后将试管球茎切片并进行包埋，得到试管球茎切片包埋珠，然后将该包埋珠接种到不同营养成分的固体培养基上进行培养，即可诱