Method for on-line synthesis of coumarin-3-carboxylic acid sugar ester derivative based on flow chemistry enzymatic catalysis

The invention relates to a method for on-line synthesis of coumarin-3-carboxylic acid sugar ester derivatives based on flow chemistry enzymatic catalysis. The method comprises the following steps: uniformly filling a reaction channel of a microfluidic channel reactor with lipase Lipozyme RM IM; respectively dissolving coumarin-3-carboxylic acid methyl ester and a carbohydrate compound by using a reaction solvent; injecting raw materials into a pipeline through a first injector and a second injector respectively and gathering; controlling the reaction temperature to be 30-60 DEG C and the continuous flowing reaction time of a mixed solution in the reaction channel to be 10-60 minutes, collecting a reaction solution flowing out of the reaction channel on line through a product collector, andperforming post-treatment to obtain a coumarin-3-carboxylic acid sugar ester derivative product, the method has the advantages of short reaction time, high yield and good selectivity. 一种基于流动化学酶促在线合成香豆素-3-羧酸糖酯衍生物的方法：将脂肪酶Lipoz