Method for determining content of tetrahydropalmatine and tetrahydroberberine chiral enantiomers in rhizoma corydalis extracting solution

The invention discloses a method for determining the content of tetrahydropalmatine and tetrahydroberberine chiral enantiomers in a rhizoma corydalis extracting solution, which belongs to the technical field of traditional Chinese medicine component content determination. According to the method, capillary electrophoresis-mass spectrometry combined analysis is adopted, a partial filling technologyis adopted, hydroxypropyl-beta-cyclodextrin is used as a chiral selective agent, and the content of the tetrahydropalmatine and tetrahydroberberine paired chiral enantiomers is directly determined ina complex sample system of the rhizoma corydalis extracting solution. The method has the advantages of simplicity and convenience in operation, high sensitivity, low detection cost (less consumptionof samples, chiral selectors and organic solvents) and high practicability; the content of the tetrahydropalmatine and tetrahydroberberine two-pair chiral enantiomers can be directly determined in a complex sample system of the