Microfluidic nucleic acid testing card box and kit

The invention is applicable to the technical field of nucleic acid testing, and provides a microfluidic nucleic acid testing card box and kit. The card box comprises a card box support, a reagent operation part, a soft connecting part, a flow path part and an amplification chamber, wherein the reagent operation part is arranged at the upper part of the interior of the card box support and is provided with a sample cracking cavity, a plurality of liquid storage cavities and a pump operation cavity; the reagent operation part is provided with a first micro-flow pipeline and a second micro-flow pipeline; one end of the first micro-flow pipeline is connected with the pump operation cavity; a third micro-flow pipeline and a plurality of fourth micro-flow pipelines are arranged on the flow pathpart; the amplification cavity is arranged below the flow path part; and a valve assembly on the soft connecting part can control the connection or disconnection between the pump operation cavity andthe sample cracking cavity