No-clean cell membrane targeting fluorescent probe and preparation method and application thereof

The invention discloses a no-clean cell membrane targeting fluorescent probe and a preparation method and application thereof. Cheap carbazole monoaldehyde derivatives and 4-methylpyridinium containing sulfonate radicals are used for reaction, the compound pyridinium salt derivative Car-py with a D-pi-A conjugated structure is prepared, the fluorescent probe can be accurately positioned on a cellmembrane of a biological cell, the compound Car-py can be used for a developing test without cleaning after acting with the cell, the operation is simple, and the microstructure and dynamic change ofthe biological cell can be visually observed in real time through fluorescence microimaging. 本发明公开了一种免洗型细胞膜靶向荧光探针及其制备方法和应用，利用廉价的咔唑单醛衍生物和含有磺酸根的4-甲基吡啶盐反应，制备出具有D-π-A共轭结构的化合物吡啶盐衍生物Car-py，其可精准地定位于生物细胞的细胞膜，化合物Car-py与细胞作用后，无需清洗即可用于显影测试，操作简单，通过荧光显微成像可实时地可视化观测生物细胞的微观结构和动态变化。