Recombinant rabies virus vaccine strain and preparation method thereof

The invention discloses a recombinant rabies virus vaccine strain and a preparation method thereof and belongs to the technical field of animal vaccine. The vaccine strain takes an attenuate vaccine SAD-B19 as a parent toxic strain, aspartic acid at the 194 site of the toxic strain is mutated into serine, the arginine at the 333 site is mutated into glutamic acid, and a murine OX40L gene is inserted between a G gene and an L gene of a genome of the ttenuate vaccine SAD-B19. The safety of the recombinant rabies virus vaccine strain is high, the recombinant rabies virus vaccine strain has safe,low-toxicity, long-term and effective characteristics and can serve as a candidate strain of a rabies virus vaccine; and compared with vaccine strains used for immunization of wild animals in Europe,namely, female parent strain LBNSE in an experiment, the recombinant rabies virus vaccine strain can rapidly induce to produce a significantly high neutralization antibody level for up to 56 days. 本发明公开一种重组狂犬病病毒疫苗株及其制备方法，属于动物疫