Squalene engineering strain, squalene synthetic plasmid, cell membrane space expansion plasmid and preparation methods

The invention relates to a squalene engineering strain, a squalene synthetic plasmid, a cell membrane space expansion plasmid and preparation methods. By constructing an engineering strain of a squalene synthetic pathway, developing a microbial cell factory and constructing a squalene synthetic unit of a gene related to membrane area expansion, the biosynthesis and industrial production process ofsqualene is promoted. According to the squalene engineering strain, the squalene synthetic plasmid and the preparation methods thereof, a green synthetic method for synthesizing the squalene in escherichia coli can reduce dependence on natural resources and overdevelopment. Moreover, the method is relatively low in cost, can realize large-scale squalene production, and has huge economic benefits. 本发明涉及一种角鲨烯工程菌株、角鲨烯合成质粒、细胞膜空间扩展质粒及制备方法，通过构建角鲨烯合成途径的工程菌株，开发微生物细胞工厂，构建与膜面积扩展相关基因的角鲨烯合成单元，从而推进角鲨烯的生物合成和工业化生产进程。该角鲨烯工程菌株、角鲨烯合成质粒及其制备方法在大肠杆菌中合成角鲨烯的绿色合成方法，可以减少对自然资源的依赖和过度开发。并且，其成本较低，能实现大规模的角鲨烯生产，具有巨大的经济效益。