Method for establishing influence of amoxicillin concentration on lactobacillus acidophilus based on metabonomics

The invention discloses a method for establishing influence of amoxicillin concentration on lactobacillus acidophilus based on metabonomics, which comprises the following steps: carrying out liquid chromatography-mass spectrometry analysis on intracellular metabolites of lactobacillus acidophilus after administration of amoxicillin with different concentrations to obtain fragment information of the metabolites; screening the fragment information of the metabolite by establishing an orthogonal partial least squares discriminant analysis model to obtain a potential biomarker, wherein the screening conditions are that the variable importance projection value is greater than 1, the difference multiple absolute value is greater than 1, and the p value is less than 0.05; performing metabolic pathway enrichment on the potential biomarkers to obtain related important metabolic pathways for occurrence and development of lactobacillus acidophilus metabolic disorders caused by amoxicillin with different concentrations;