Non-replicative transduction particles and transduction particle-based reporter systems

Methods and systems are provided for packaging reporter nucleic acid molecules into non- replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non- replicative transduction particles as reporter molecules. 提供了用于将报告核酸分子包装到非复制型转导颗粒内，用作报告分子的方法和系统。非复制型转导颗粒可以由病毒构建，并且使用病毒转导和复制系统。报告核酸分子包括用于检测靶基因或细胞的报告基因，例如报告分子或可选择标记物。提供了使用非复制型转导颗粒作为报告分子用于检测细胞和靶核酸分子的方法和系统。