Chemiluminiscence detection kit for S100B protein and using method of chemiluminiscence detection kit

The invention is applicable to the field of biotechnology, and particularly relates to a chemiluminiscence detection kit for S100B protein and a using method of the chemiluminiscence detection kit. The detection kit of the S100B protein comprises an elisa plate coated with an anti-S100B monoclonal antibody, a horse radish peroxidase labeled anti-S100 polyclonal antibody solution for detection, a standard substance, a standard substance diluent, a sample diluent, a concentrated washing solution, a luminescent solution A, a luminescent solution B and a detection antibody diluent. According to the kit provided by the embodiment of the invention, the reaction time of a to-be-detected product (a standard product or a serum sample) and the horse radish peroxidase labeled anti-S100 polyclonal antibody solution for detection is 2 hours; clinically common pathological jaundice and lipidemia specimens have no obvious interference on serum S100B protein determination; and both the intra-batch mean variation and inter-ba