Method for detecting titer of antivenin

The invention relates to the technical field of biological medicines, in particular to a method for detecting the titer of antivenin. The method comprises the following steps: loading the antivenin onto an affinity chromatographic column, performing immunoaffinity adsorption reaction with snake venom, and sequentially eluting to obtain an uncombined snake venom component and a combined snake venomcomponent; and analyzing the binding amount of the antivenin to each component of the snake venom according to the HPLC detection result. According to the method, animal welfare can be improved, andvisualization of antivenin titer evaluation can be achieved. 本发明涉及生物医药技术领域，尤其涉及抗蛇毒血清效价的检测方法。该方法包括：将抗蛇毒血清上亲和层析柱后，与蛇毒进行免疫亲和吸附反应，依次洗脱获得未结合蛇毒组分、结合蛇毒组分；根据HPLC检测结果分析抗蛇毒血清对蛇毒各组分的结合量。该方法可以改善动物福利，且能够实现蛇毒血清效价评价的可视化。