Primer composition for detecting Y chromosome microdeletion and sex chromosome number and application

The invention relates to the field of molecular biology diagnosis, in particular to a primer composition for detecting Y chromosome microdeletion and the sex chromosome number and an application. A Ychromosome AZF region sequence marker site and a sex chromosome number detection site can be simultaneously amplified by utilizing special primer design in a tube of PCR reaction solution; a male sexdetermination gene (SRY) is used as a sex abnormality control and a human X/Y linked zinc finger protein gene (ZFX/Y) is used as an internal control; PCR products are analyzed through capillary electrophoresis; and whether an AZF region of Y chromosome is deleted or not and whether the sex chromosome number is abnormal or not are interpreted by comparing the PCR products with different fragment sizes. A result can be obtained within 3 hours from a DNA sample, the Y chromosome microdeletion and the Klinefelter syndrome can be detected at the same time only through one PCR experiment, the detection technical process is s