Preparation, separation, purification and identification method of cucurbita pepo seed oxidation resisting peptide

The invention discloses a preparation, separation, purification and identification method of cucurbita pepo seed oxidation resisting peptide. The preparation, separation, purification and identification method is characterized by comprising the steps of adding cucurbita pepo seed powder to water, adding enzymes, performing enzymolysis, performing enzyme deactivation, and removing an oil layer to obtain cucurbita pepo seed meal; using the cucurbita pepo seed meal as a substrate, adding the enzymes, performing freeze drying on supernatant after centrifugation of enzymolysis products, and obtaining a cucurbita pepo seed oxidative peptide resisting mixture; dissolving the cucurbita pepo seed oxidative peptide resisting mixture in deionized water, performing redissolution, adopting membrane bags of different relative molecular mass, controlling the outlet pressure of an ultra filtration membrane plate, after ultrafiltration, collecting obtained components, naming the obtained components according to different rela