Technology for producing porcine reproductive and respiratory syndrome viruses by Marc-145 cells cultured in microcarrier

The invention provides a technology for producing porcine reproductive and respiratory syndrome viruses by Marc-145 cells cultured in a microcarrier. The technology comprises the following technique steps of selecting a bioreactor as a culture means: selecting parameters of temperature, PH, dissolved oxygen, stirring speed and the like which can be automatically controlled, and selecting the bioreactor suitable for suspension culture in the microcarrier. The microcarrier is selected as a carrier allowing cells to be attacked for growth, namely a Cytodex series microcarrier; and cleaning the microcarrier and performing sterilization. In order to overcome defects of a conventional rotating bottle method, the invention provides the production technology for producing porcine reproductive andrespiratory syndrome viruses by Marc-145 cells cultured in the microcarrier. According to the technology, the production cost can be greatly reduced, the space occupied is small, production scale canbe quickly enlarged, the e