CD61 protein resisting monoclonal antibody, cell line and preparation method and application of CD61 protein resisting monoclonal antibody

The invention relates to the field of biological detection, and provides a CD61 protein resisting monoclonal antibody. The amino acid sequences of a heavy chain variable region and a light chain variable region of the CD61 protein resisting monoclonal antibody are respectively amino acid sequences a...

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Bibliographische Detailangaben
Hauptverfasser:	WANG XIAOYA, YANG QINGHAI, JIANG NINGCHENG, FU LILI, ZHOU HONGHUI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of biological detection, and provides a CD61 protein resisting monoclonal antibody. The amino acid sequences of a heavy chain variable region and a light chain variable region of the CD61 protein resisting monoclonal antibody are respectively amino acid sequences as shown in SEQID NO.2 and SEQID NO.3. The inventor further provides a preparation method of the CD61protein resisting monoclonal antibody. An antigen used for immunization of mice is nucleotide sequence codes as shown in SEQID NO.1. The invention provides recombination proteins subjected to recombinant expression with escherichia coli. The inventor further provides a hybridoma cell line for secreting CD61 resisting proteins, the cell line is a mouse hybridoma cell line AbM58016-19H11-PU, and the preservation No. is CGMCC NO.17490. The CD61 resisting protein monoclonal antibody has high specificity and sensibility, can specially recognize cells for expression of the CD61 protein, and is suitable for immunology detec