HOLLOW FIBER CELL CULTURE DEVICE, CELL CULTURE METHOD, AND METHOD FOR PRODUCING CULTURE SUPERNATANT

The invention is to provide a culture device. A hollow fiber cell culture device for producing a culture supernatant, said culture device comprising: a filter housing 1; hollow fibers 3 existing in the filter housing and having a pore size of from 5 nm to 1 [mu]m inclusive, into which cells are intr...

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Hauptverfasser: URUSHIHATA NAOKI, OKI KATSUYUKI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention is to provide a culture device. A hollow fiber cell culture device for producing a culture supernatant, said culture device comprising: a filter housing 1; hollow fibers 3 existing in the filter housing and having a pore size of from 5 nm to 1 [mu]m inclusive, into which cells are introduced via a cell introduction part 5; a first end cap 6a for sealing a first end 4a of the hollow fibers 3 and the filter housing 1; a culture liquid introduction part 7 for introducing a culture liquid into the filter housing; and a culture liquid discharge part 9 for discharging the culture liquid having passed through the inside of the filter housing. 本发明的课题是提供一种培养装置。本发明的解决手段是一种用于制造培养上清液的中空纤维细胞培养装置,具有:过滤器外壳1;中空纤维3,其存在于过滤器外壳的内部,孔径是5nm以上且1μm以下,通过细胞导入部5而将细胞导入内部;中空纤维3的第一末端4a;第一端帽6a,其用于密闭过滤器外壳1;培养液导入部7,其用于将培养液导入过滤器外壳的内部;以及培养液排出部9,其用于将经过过滤器外壳的内部的培养液排出。