PCR-HRM (Polymerase Chain Reaction-High Resolution Melting) primer for authenticating swine acute diarrhoea syndrome virus and porcine epidemic diarrhea virus, method for authenticating swine acute diarrhoea syndrome and porcine epidemic diarrhea, and application of PCR-HRM primer

The invention discloses a PCR-HRM (Polymerase Chain Reaction-High Resolution Melting) primer for authenticating a swine acute diarrhoea syndrome virus and a porcine epidemic diarrhea virus, a method for authenticating a swine acute diarrhoea syndrome and porcine epidemic diarrhea, and application of the PCR-HRM primer. A primer pair provided by the invention has good specificity, only one pair ofprimers needs to be used for carrying out PCR amplification on the SADS-CoV (Swine Acute Diarrhoea Syndrome Coronavirus) and the PEDV (Porcine Epidemic Diarrhea Virus), a bonding situation of a double-stranded DNA fluorescent dye and a PCR amplification product in a temperature rise process can be monitored in real time, and fluorescent data is collected and is authenticated according to the difference of two virus melting curves. The detection method provided by the invention does not need the separation and identification of viruses, virus authentication time is shortened, gel electrophoresis is not required for obs