MiRNA-128-3p of targeting Galectin-3 gene and use of miRNA-128-3p to resistance of pancreatic cancer

The invention discloses miRNA-128-3p of targeting a Galectin-3 gene. A sense strand of the miRNA-128-3p is 5'-CACUAGAUUGUGAGCUCCUGGA-3', and an antisense strand of the miRNA-128-3p is 5'-UCCAGGAGCUCACAAUCUAGUG-3'. The invention further discloses a drug carrier for the miRNA-128-3...

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Bibliographische Detailangaben
Hauptverfasser:	XUAN SHIHAI, XIE XUDONG, LI MEI, LU CUIHUA, XIAO MINGBING, JIANG FENG, PAN LINGLING, WANG ZHENGDONG, CHEN FEI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses miRNA-128-3p of targeting a Galectin-3 gene. A sense strand of the miRNA-128-3p is 5'-CACUAGAUUGUGAGCUCCUGGA-3', and an antisense strand of the miRNA-128-3p is 5'-UCCAGGAGCUCACAAUCUAGUG-3'. The invention further discloses a drug carrier for the miRNA-128-3p of targeting the Galectin-3 gene, and the drug carrier is an exosome (EXO) derived from human umbilical cord mesenchymal stem cells (HUCMSCs). In addition, the invention further discloses experimental verification of use of the miRNA-128-3p, carried by the HUCMSCs exosome, of targeting the Galectin-3 gene to resistance of the pancreatic cancer. According to the embodiments of the invention, miRNA-128-3p mimic is successfully transfected to a neonatal umbilical cord tissue-derived HUCMSCs-EXO to construct an engineered EXO; and the engineered EXO can inhibit migration, invasion and proliferation of pancreatic cancer cells by targeting Galectin-3. 本发明公开一种靶向Galectin-3基因的miRNA-128-3p，所述miRNA-128-3p的正义链：5'-CACUAGAUUGUGAGCUCCUGGA-3'，其反义链：