Display substrate, preparation method thereof and display device

The embodiment of the invention provides a display substrate, a preparation method thereof and a display device, which relate to the technical field of display, and can improve the quality and the display effect of the display device. The display substrate is provided with a display area and a packaging area surrounding the display area. The display panel comprises a substrate and a spacer layer.The spacer layer is located on one side of the substrate, the spacer layer comprises a plurality of first columnar spacers which are located in the packaging area and distributed around the display area at intervals, and the bottom ends of the first columnar spacers are fixedly arranged on the substrate; the ends, close to the substrate, of the first columnar spacers are the bottom ends of the first columnar spacers, and the ends, away from the substrate, of the first columnar spacers are the top ends. 本公开的实施例提供一种显示用基板及其制备方法、显示装置，涉及显示技术领域，能够改善显示装置的品质和显示效果。其中，显示用基板具有显示区和围绕显示区的封装区。显示面板包括衬底和隔垫物层。隔垫物层位于衬底的一侧，隔垫物层包括位于封装区且围