High performance liquid chromatography-tandem mass spectrometry determination method of rhodoxanthin in red-heart duck eggs

The invention discloses a high performance liquid chromatography-tandem mass spectrometry determination method of rhodoxanthin in red-heart duck eggs, and belongs to the technical field of analysis and detection. A quantitative detection method of the rhodoxanthin in the red-heart duck eggs is established by utilizing high performance liquid chromatography tandem mass spectrometry (LC-MS/MS). Themethod comprises the following steps: extracting a red-heart duck egg sample with acetonitrile; carrying out rotary evaporation and concentration; and degreasing with n-hexane, and determining on a machine. Results show that the method is simple to operate, high in sensitivity and good in accuracy, a method reference is provided for determination of the rhodoxanthin in the red-heart duck eggs, andthe method has a great application potential and economic value in the fields of red-heart duck egg processing and production, quality grading and the like. 本发明公开了一种红心鸭蛋中紫衫紫素的高效液相色谱串联质谱测定方法，属于分析检测技术领域。本发明通过利用高效液相色谱串联质谱(LC-MS/