Closed-tube nucleic acid amplification detection method and device

The invention discloses a closed-tube nucleic acid amplification detection method and device. The closed-tube nucleic acid amplification detection method comprises the steps: nucleic acid is extractedfrom a sample, the extracted nucleic acid serves as a template to be mixed with an amplification reagent, a reaction system is obtained, meanwhile the reaction system and a detection system are placed into a reaction tube, and the reaction system and the detection system are separated through a detection tube in the reaction tube to guarantee that the reaction system and the detection system do not make contact with each other before an amplification reaction is completed; and after the amplification reaction is completed, through reverse shaking, a reaction product and the detection system are evenly mixed, and finally visual observation shade matching and analysis are conducted. The acid amplification and result analysis processes are completed under a closed-tube condition, and the advantages that the detectio