Liquid phase staining identification method used for cells and needing extremely small cell quantity

The invention provides a liquid phase staining identification method used for cells and needing extremely small cell quantity. The method comprises the following steps: (1) adding a cell immobilizingsolution into cells in a non-dry state for cell immobilization; (2) after cleaning the cells, adding a cell staining solution for cell staining, wherein the cell staining solution comprises a fluorescent antibody staining solution; (3) dyeing the cells by utilizing bright-field light dyeing liquid; and (4) after cleaning the cells, suspending the cells, and observing the cells under fluorescence and bright-field light via a microscope so as to simultaneously obtaining various fluorescence and bright-field light images. According to the invention, on the premise that cells are not dried or sealed, staining and imaging via dual light sources of fluorescence antibody and bright field optical cell morphology can be carried out, and high-quality fluorescence markers and bright-field optical morphological images of the