Strain for preparing rapamycin derivatives through microbial transformation and application of strain

The invention relates to the field of microorganisms, in particular to a strain for preparing rapamycin derivatives through microbial transformation and application of the strain. The strain is Bacillus subtilis F9, is preserved in the China General Microbiological Culture Collection Center on June 19, 2013, and has a preservation number of CGMCC NO. 7764. The strain is applied to preparation of the rapamycin derivatives through transformation. The strain has a transformation effect on rapamycin, rapamycin is adopted as a substrate by the strain, and a C1 product is obtained after cultivation;and it is found through nuclear magnetic analysis that the structure of the C1 product is 43-beta-D-glucopyranose-rapamycin, and the C1 product has an anti-tumor effect. 本发明涉及微生物领域，尤其涉及一种微生物转化制备雷帕霉素衍生物的菌株及其应用。所述菌株为枯草芽孢杆菌(Bacillus subtilis)F9，保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏日期为2013年06月19日，保藏编号为CGMCC NO.7764。所述菌株用于转化制备雷帕霉素衍生物。本发明获得了一株对雷帕霉素具有转化作用的细菌，该菌以雷帕霉素为底物，经培养得到C1产物,经核磁解析C1结构为43-β-D-吡喃葡萄糖-雷帕霉素，具有抗肿瘤作用。