Application of osteopontin as target molecule in regulation of intestinal flora colonization

The invention discloses an application of osteopontin as a target molecule in regulation of intestinal flora colonization. Specifically, the method comprises the following steps that OPN gene expression is inhibited or combination of OPN and a cell receptor is blocked so as to increase the number of probiotics in intestinal tracts; blocking combination of the OPN and the cell receptor or inhibition of a Notch signal path can improve the adhesion effect of bacteria and intestinal epithelial cells. The method is advantaged in that the method proves that the osteopontin influences the adhesion effect between cells and the bacteria by inhibiting the expression of cell adhesion factors, and thereby colonization of flora in intestinal tracts is inhibited. 本发明公开了骨桥蛋白作为靶分子在调节肠道菌群定植中的应用；具体为：抑制OPN基因表达或阻断OPN与细胞受体结合，从而提高肠道中益生菌的数量；阻断OPN与细胞受体的结合或抑制Notch信号通路能提高细菌与肠上皮细胞的黏附作用。本发明证实了骨桥蛋白通过抑制细胞粘附因子的表达影响细胞与细菌之间的黏附作用，进而抑制了菌群在肠道中的定植。