Method for activating Cas14a enzyme supplementary cleavage effect by RNA

Method for activating Cas14a enzyme supplementary cleavage effect by RNA

The invention can be used in the fields of analysis, detection and gene editing, and can accordingly provide a method for activating Cas14a ''supplementary cleavage'' activity into a non-specific ssDNase by RNA to solve the problem that the RNA cannot activate the supplementary c...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: WAN YI, YANG ZHIQING, WEI YANGDAO
Format: Patent
Sprache:chi ; eng
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BIOCHEMISTRY
CHEMISTRY
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CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
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Beschreibung
Zusammenfassung:The invention can be used in the fields of analysis, detection and gene editing, and can accordingly provide a method for activating Cas14a ''supplementary cleavage'' activity into a non-specific ssDNase by RNA to solve the problem that the RNA cannot activate the supplementary cleavage activity of a Cas14a enzyme and expand an application range of the Cas14a enzyme from DNA to RNA. 本发明可用于分析检测及基因编辑领域，能够相应的提供一种RNA激活Cas14a"附带切割"活性即可被激活成为非特异性的ssDNase的方法，解决RNA不能激活Cas14a酶的附带切割活性的问题，拓展Cas14a酶由DNA到RNA的应用范围。