ENZYME SCREENING METHODS

The present disclosure is directed to compositions and methods for deriving a plurality of kinetics parameters (240) for at least two different enzyme variants in a multiplex manner using nanopore-based sequencing. In some embodiments, the systems and methods may be used to screen different nanopore...

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Bibliographische Detailangaben
Hauptverfasser:	PALLA MIRKO, AYER ARUNA, STRANGES PETER B, PUNTHAMBAKER SUKANYA SRINIVASA RAO ARUN, CHURCH GEORGE M, PEPIN FRANCIOS
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure is directed to compositions and methods for deriving a plurality of kinetics parameters (240) for at least two different enzyme variants in a multiplex manner using nanopore-based sequencing. In some embodiments, the systems and methods may be used to screen different nanopore variants, or different combinations of both nanopore variants and enzyme variants. 本发明涉及使用基于纳米孔的测序以多重方式推导至少两种不同的酶变体的多种动力学参数(240)的组合物和方法。在一些实施方案中，所述系统和方法可用于筛选不同的纳米孔变体，或纳米孔变体和酶变体两者的不同组合。