White birch BpNAC100 gene, and amino acid sequence and application thereof

The invention discloses a white birch BpNAC100 gene, and an amino acid sequence and application thereof. According to the application of the BpNAC100 gene in response to salt stress of white birches,the nucleotide sequence of the BpNAC100 gene is shown as SEQ ID NO:1 in a sequence table, and the seq...

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Hauptverfasser: LIU GUIFENG, LI TIANFANG, FANG GONGGUI, JIANG JING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a white birch BpNAC100 gene, and an amino acid sequence and application thereof. According to the application of the BpNAC100 gene in response to salt stress of white birches,the nucleotide sequence of the BpNAC100 gene is shown as SEQ ID NO:1 in a sequence table, and the sequence of the amino acid is shown as SEQ ID NO:2 in the sequence table. According to the invention,overexpression and expression inhibition are separately carried out on the BpNAC100 gene and a promoter thereof in white birches by using an agrobacterium mediation method, so that a proBpNAC100::Luctransgenic birch plant and a BpNAC100::GFP, BpNAC100-RNAi transgenic birch plant are obtained. Therefore, the promoter of the BpNAC100 gene has spatial expression specificity in different tissue partsof white birches, and the BpNAC100::GFP transgenic birch plant has salt damage resistance. 本发明公开了一种白桦BpNAC100基因及其氨基酸序列和应用,BpNAC100基因在白桦响应盐胁迫上的应用,BpNAC100基因的核苷酸序列如序列表中SEQ ID NO:1所示,氨基酸的序列表如序列表中SEQ ID NO:2所示;本发明利用农杆菌介导法分别在白桦中过表达和