Disulfide analogue of pramlintide and preparation method thereof

The invention discloses a disulfide analogue of pramlintide and a preparation method thereof. The pramlintide disulfide analogue which is stable under a reduction condition is obtained by performing disulfide bond replacement on polypeptide medicine of pramlintide. The synthesis of a target polypeptide is realized by a Fmoc solid-phase synthesis method; and a crude product is purified and freeze-dried to obtain the disulfide analogue of the pramlintide. Reduction stability experiments show that the disulfide analogue of the pramlintide has good reduction stability. 本发明公开了一种普兰林肽的二硫类似物及其制备方法，通过对多肽药物普兰林肽进行二硫键的替换得到一种还原条件下稳定的普兰林肽二硫类似物。目标多肽的合成是通过Fmoc固相合成方法实现，粗品经纯化，冻干得到普兰林肽的二硫类似物。还原稳定性实验表明本发明普兰林肽的二硫类似物具有良好的还原稳定性。