Method for rapid and damage-free identification of ovum maturity of hyriopsis cumingii

The invention discloses a method for rapid and damage-free identification of the ovum maturity of hyriopsis cumingii. The method comprises the following steps: selecting hyriopsis cumingii with an ageof three or more through annual rings, opening a gap of 0.7-0.8 cm between two shells of the hyriops...

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Hauptverfasser: YUAN LI, HU HONGHUI, GUO SIPENG, FENG SHANGLE, BAI ZHIYI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for rapid and damage-free identification of the ovum maturity of hyriopsis cumingii. The method comprises the following steps: selecting hyriopsis cumingii with an ageof three or more through annual rings, opening a gap of 0.7-0.8 cm between two shells of the hyriopsis cumingii, checking the distance and number of gill filaments of inner and outer gills, screeningand selecting female hyriopsis cumingii with full gonad for marking, sucking an ovum liquid from the surface layer tissues of the gonad of the female hyriopsis cumingii by using an injector, dilutingthe ovum liquid to a volume of 1 mL, placing an obtained ovum diluent under an optical microscope, and observing ovum conditions, wherein mature ova simultaneously meet the following conditions: (1)the ova are nearly circular; (2) the ovum diameter is large; (3) a colloidal membrane with uniform thickness is arranged outside an ovum membrane; (4) yolk particles in the ovum membrane are uniformlydistributed, and are dark br