HDN staining solution and kit for specific color development of horseradish peroxidase by using HDN staining solution

The invention belongs to the technical field of cell staining and kits, and particularly relates to an HDN staining solution and a kit thereof for specific color development of horseradish peroxidaseby using the HDN staining solution. The kit comprises the HDN staining solution, wherein the HDN staining solution comprises hydrogen peroxide, a buffer solution, ammonium nickel sulfate, 3, 3'-diaminobenzidine and ammonium chloride. An immunohistochemistry method of the kit comprises the following steps of: (1) slicing and fixing a detected living tissue; 2) detecting a to-be-discovered antigen or antibody by using a primary antibody; 3) carrying out staining, specifically, immersing the obtained tissue slice into the HDN staining solution, wherein a positive detection object of the tissue slice is dark purplish blue; 4) cleaning the tissue slice; 5) and sealing the slice, and observing the tissue slice. By adopting the HDN staining solution for staining, the obtained sensitivity of the kit is more than 10 times