Colloidal gold immunochromatography kit for rapidly detecting novel coronavirus IgM antibody and preparation method thereof
The invention discloses a colloidal gold immunochromatography kit for rapidly detecting a novel coronavirus IgM antibody and a preparation method thereof, and relates to the field of biomedicine. Whether an anti-novel coronavirus nucleocapsid protein IgM antibody exists in human serum or blood plasm...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a colloidal gold immunochromatography kit for rapidly detecting a novel coronavirus IgM antibody and a preparation method thereof, and relates to the field of biomedicine. Whether an anti-novel coronavirus nucleocapsid protein IgM antibody exists in human serum or blood plasma or not is qualitatively detected by adopting an antigen-antibody sandwich method and a colloidal gold immunochromatography principle; colloidal gold is used for labeling the novel coronavirus nucleocapsid protein containing the 6*His label to be combined to form gold-labeled N protein, and then the gold-labeled N protein is adsorbed on the gold-labeled pad; novel coronavirus nucleocapsid protein is used as an indication marker, the detection line of the NC membrane is coated with a mouse anti-human u chain monoclonal antibody, and the quality control line of the NC membrane is coated with a mouse anti-6*His monoclonal antibody; and qualitative detection of the anti-novel coronavirus nucleocapsid protein IgM antib |
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