Colloidal gold immunochromatography kit for rapidly detecting novel coronavirus IgM antibody and preparation method thereof

The invention discloses a colloidal gold immunochromatography kit for rapidly detecting a novel coronavirus IgM antibody and a preparation method thereof, and relates to the field of biomedicine. Whether an anti-novel coronavirus nucleocapsid protein IgM antibody exists in human serum or blood plasm...

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Hauptverfasser: LUO JUNLAN, LI MINYI, MA YULAN, WEI JIANHUA, WANG SHENGLAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a colloidal gold immunochromatography kit for rapidly detecting a novel coronavirus IgM antibody and a preparation method thereof, and relates to the field of biomedicine. Whether an anti-novel coronavirus nucleocapsid protein IgM antibody exists in human serum or blood plasma or not is qualitatively detected by adopting an antigen-antibody sandwich method and a colloidal gold immunochromatography principle; colloidal gold is used for labeling the novel coronavirus nucleocapsid protein containing the 6*His label to be combined to form gold-labeled N protein, and then the gold-labeled N protein is adsorbed on the gold-labeled pad; novel coronavirus nucleocapsid protein is used as an indication marker, the detection line of the NC membrane is coated with a mouse anti-human u chain monoclonal antibody, and the quality control line of the NC membrane is coated with a mouse anti-6*His monoclonal antibody; and qualitative detection of the anti-novel coronavirus nucleocapsid protein IgM antib